PeptiLine
PeptiLine: an interactive platform for customizable functional peptidomic analysis

Process raw peptidomic search results, or bring in data that is already annotated with functional information, then organize, analyze, and visualize your dataset to surface insight and generate publication-ready figures.

3
Integrated analysis modules
6
Step end-to-end workflow
13
Exportable data files
14
Analysis & visualization views

Tools

Three integrated modules

Each tool builds on the last: organize your data once, then analyze and visualize it as many ways as you need.

Data Transformation upload screen

Data Transformation

Organize peptidomic data, whether raw search results or data already annotated with functional information, and prepare it for analysis.

  • Multi-dataset import and merge
  • Functional annotation, including MBPDB and custom reference sets
  • Study variable and group assignment
  • Thirteen exportable file types, from merged data to correlations and protein maps
Start Data Transformation
Data Analysis dashboard

Data Analysis

Descriptive and correlative analysis of your dataset, with statistical significance testing to compare sample groups.

  • Grouped, stacked, pie, and correlation scatter plots
  • Replicate-based error bars and ANOVA/Tukey HSD or Welch/Games-Howell significance testing
  • Adjustable scaling and orientation
  • Customizable color palettes, titles, and axis and legend labels
Explore Data Analysis
Sequence Heatmap dashboard

Heatmap Visualization

Map peptide abundance and counts onto protein sequences to reveal site-specific and functional hotspots, with statistical comparison between groups.

  • Site-specific peptide abundance and count mapping
  • Differential comparison (standardized mean difference or log2 fold-change) between groups or protein variants
  • Landscape, portrait, and compact layouts
Create Heatmaps

Visualizations

Explore plot types

Every plot is interactive and exportable. Click any example to view it full size.

Stacked Bar Plot
Stacked Bar Plots
Absolute and relative abundance distributions within and across samples, proteins, or functional categories.
Sequence Heatmap
Sequence Heatmaps
Site-specific peptide abundance and counts mapped across one or more protein sequences.
Pie Plot
Pie Charts
Relative contribution by sample, functional category, or protein origin.
Protein Plot
Grouped Bar Plots
Peptide abundance by protein origin or function, compared across samples, with replicate-based error bars.
Summary Bar Plot
Summary Bar Plots
Total peptide counts and abundance summaries with statistical error bars.
Scatter Plot
Correlation Scatter Plots
Sample-to-sample correlation, including SPLOM plotting options.

Statistical comparison

Compare groups with statistical rigor

Significance testing and differential effect-size views for comparing sample groups.

Bar plot with compact-letter significance groupings
Compact-Letter Significance Bars
Replicate-based error bars with ANOVA/Tukey HSD (or Welch/Games-Howell) results shown as compact-letter groupings across three or more sample groups.
Differential comparison heatmap
Differential Comparison Heatmap
Standardized mean difference or log2 fold-change between two groups, overlaid on the sequence heatmap.
Bar plot with two-group significance bracket
Two-Group Significance Brackets
Replicate-based error bars with an ANOVA/Tukey HSD (or Welch/Games-Howell) two-group comparison shown as an asterisk-style bracket between the compared bars.

Scaling & orientation

Adjust how abundance is represented

The same dataset, viewed as absolute totals or relative contributions.

Absolute Summed Plot
Absolute Summed Abundance
Total peptide contribution by protein origin or function across groups.
Relative Protein Plot
Relative Abundance by Protein
Protein or function contribution percentages across samples.
Relative Sample Plot
Relative Abundance by Sample
Sample-specific protein or function contributions.

Ready to analyze your data?

Start with Data Transformation to organize and annotate your peptidomic dataset.