PeptiLine
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Jump right into analyzing your peptidomic data with our powerful tools

Data Transformation Data Analysis Heatmap Visualization

To learn more about the process, see the workflow below and explore our visualization examples

⚙️ Workflow

Data Transformation
Organize, label, annotate and explore your peptidomic data with the data transformation tool

Features

  • Automated peptide-to-protein mapping
  • MBPDB bioactivity annotation
  • Study variable assignment
  • Exploritoy data analysis
  • Multiple data export options

Inputs

  • Peptidomic data
  • Functional data (optional)
  • Protein FASTA file (optional)

Steps

  • Upload files and functional annotation
  • Organize peptides with multiples protein assignments
  • Assign study variables or grouppings
  • Process and export datasets

Exports

  • Merged dataset (for future visualizations)
  • Functional data (raw and summarized)
  • Variable definitions (JSON)
  • Correlations within and between samples
  • Total peptide count and abundance summaries
  • Protein contribution of peptides
  • List of peptides by samples
Start Data Transformation
Descriptive Analysis
Explore you data and generate visualizations through interactive plots

Features

  • Perform descriptive analysis
  • Functional profiling & visualization
  • Protein origin contribution analysis
  • Transform and visualize your data
  • Adjust data scaling and orientation
  • Customizable appearance settings
  • Generate interactive plots

Inputs

  • Merged data (from Data Transformation)

Steps

  • Select sample(s) to analyze
  • Apply data filter (protein or functional)
  • Select plot type and settings
  • Generate, explore and export plots

Exports

  • Interactive Plottly plots (HTML)
  • Static image exports (PNG)
Explore Data Analysis
Sequence Visualization
Generate heatmap visualization mapping site specific peptide absorbance and count along protein sequences

Features

  • Protein sequence mapping
  • Visualize peptide absorbance and count
  • Functional peptide highlighting
  • Comparative sample analysis
  • Compare different protein sequences
  • Customizable color and label settings
  • Functional hotspot identification

Inputs

  • Merged data (from Data Transformation)

Steps

  • Select sample(s) to analyze
  • Select protein(s) to analyze
  • Filter data
  • Select plot options
  • Generate, explore and export plots

Exports

  • Static image exports (PNG)
Create Heatmaps

📈 Visualizations

Explore Different Plot Types

Discover the best visualization formats to easily reveal insights from your data

Scaled Plot
Click to view interactive plot
Stacked Bar Plots
Easily visualize both absolute and relative abundance distributions to understand the trends within and across samples proteins or functional categories
Sequence Heatmap
Click to expand the figure
Sequence Heatmaps
Identify key regions of peptide or functional distribution with in a protein(s) illustrating site specific peptide absorbance and peptide counts
Pie Plot
Click to view interactive plot
Pie Charts
Identify relationships within each sample, functional categories or protein origin with interactive pie charts
Protein Plot 1
Click to view interactive plot
Grouped Bar Plots
Grouped bar plots showing peptide abundance by protein origin (or function) across samples
Summary Bar Plot
Click to view interactive plot
Summary Bar Plots
Total peptide counts and abundance summaries with statistical error bars to understand the overall distribution of your data
Scatter Plot 1
Click to view interactive plot
Correlation Scatter Plots
Identify correlations between samples with our interactive scatter plots and SPLOM ploting options

Explore Data Scaling and Orientations Options

Discover insights through diverse data transformations and visualization options

Absolute Summed Plot
Click to view interactive plot
Absolute Summed Abundance
Absolute abundance distribution comparing the total peptide contribution by protein orgin or function across groups and relative contribution within each group
Relative Protein Plot
Click to view interactive plot
Relative Abundance by Protein
Relative abundance distribution showing protein (or function) contribution percentages across samples
Relative Sample Plot
Click to view interactive plot
Relative Abundance by Sample
Relative abundance distribution showing sample-specific protein (or function) contributions

Get Started to Explore Even More Visualization Options

Ready to dive deeper? Access our full suite of customizable interactive visualization tools

Data Transformation Data Analysis Heatmap Visualization